Cell mechanics and neurodegeneration lab
News
Research Topics
We are a new lab in the Interfaculty Institute for Biochemistry (IFIB) at the University of Tübingen in Germany.
We are interested in neuronal cell biology, with an emphasis on the cytoskeleton-membrane interactions in neurodegenerative diseases such as Alzheimer's and Parkinson's.
The vast majority (>90%) of patients clinically diagnosed with Alzheimer’s or Parkinson’s diseases (AD, PD) do not have a clear genetic etiology. Research in the last decades have identified multiple candidate genes causal for the development of early-onset forms of these diseases, yet little is known on what triggers the late-onset forms. How can we correlate known early-onset genes with unknown triggers of the late-onset forms? Are there intersections where non-genetic factors directly influence cellular processes leading to the observed pathological hallmarks of neurodegeneration?
Our work will leverage insights from genetic and physical processes in cells to understand how the relationship between biochemical and mechanical deficits converge and influence the course of neurodegeneration. We hope to take advantage of new data including non-genetic views that will require new concepts and advanced biophysical tools to corroborate late-onset markers (e.g. protein aggregates) with genetic origins of the diseases.
Schematic diagram summarizing past and on-going works using iPSC-derived cortical neurons to elucidate the interplay between axonal transport and cytoskeleton architecture. (Rafiq NM in submission, Rafiq et al 2022, Gowrishankar et al 2021).
We used a previously described synaptic vesicle-like reconstitution system in fibroblast to model the different secretory vesicle pools of iPSC-derived dopaminergic neurons. Dopamine-containing vesicles are strikingly larger than the glutamate-containing counterparts. These findings may have implications in understanding non-classical synapses, and the preferential vulnerability of dopaminergic synapses in Parkinson’s disease.
Fujise et al 2024. (BioRxiv preprint)
Schematic diagram summarizing the impact of Parkinsonism synaptojanin-1 mutant in primary cilia of iPSC-derived dopaminergic neurons (Rafiq et al 2024). Current follow-up works include connecting ciliary calcium levels and pacemaker-related functions.
We have previously described a role for bridging proteins (e.g. KANKs) in regulating cell adhesion turnover by connecting actomyosin-dependent contractility and microtubules (Rafiq et al 2019a, Rafiq et al 2019b). We are excited to explore these mechanisms in neurons in the future!
The Team
Nisha Mohd Rafiq, PhD
Group Leader / Principal Investigator
Cell Mechanics & Neurodegeneration Lab
University of Tübingen
Postdoc - Neuroscience @ Yale University
Advisor: Pietro De Camilli
PhD - Mechanobiology @ National University of Singapore & King's College London
Advisors: Sasha Bershadsky (primary), Gareth Jones & Paul Matsudaira
Lab members
Polina Volos (GTC Neuroscience)
Jaya Mishra (University of Tübingen)
Anuskhka Dutta (University of Tübingen)
Melissa Lang-Schnee (University of Tübingen)
Karmali Nasser (Jan 2025, University of Tübingen)
Ke Wang (Bachelor student)
Barbara Birk (Technician with Feil Group)
Ladina Müller (Secondary supervisor with Philipp Kahle, HIH)
ALUMNI
Makuan AbdelWahed (Bachelor student)
Beyza Ileili (Erasmus student)
If you are interested in joining our team or fund our research on Alzheimer's & Parkinson's,
please reach out via the contact information below.
Institute and Tübingen
Interfaculty Institute for Biochemistry
Faculty of Mathematics and Sciences
University of Tübingen
Auf De Morgenstelle 34
Tübingen, Germany 72076
Office: 4A36 (4th floor)
Lab: 5th floor
Contact us
Interested in joining the lab?
We value people from diverse backgrounds, regardless of race, religious beliefs, ethnic origins, gender identities, socioeconomic statuses and sexual orientations.
Scientifically, we welcome people with the following expertise: bioengineering, physics, computer science, cell biology and/or neuroscience to be part of our multidisciplinary environment. We are actively exchanging ideas with the local community and our international collaborators (Singapore, USA). We strongly encourage applications from abroad for our graduate and postgraduate positions.
Undergraduates, Masters and PhD
Please send me your CV with reference contact information. If you are currently a student in Tübingen, you can send me a quick email and we can have a chat at IFIB.
Postdocs
Please send me an email outlining your past work and future interests, together with your CV.